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| Rivera-ingraham, Georgina A.; Barri, Kiam; Boel, Melanie; Farcy, Emilie; Charles, Anne-laure; Geny, Bernard; Lignot, Jehan-herve. |
| Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H(2)DFFDA). Further analyses considered anterior and posterior gills... |
| Tipo: Text |
Palavras-chave: Antioxidant defense; Carcinus aestuarii; ROS production; Osmoregulation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73816/74410.pdf |
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| Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
| Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
| Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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| Theuerkauff, Dimitri; Rivera-ingraham, Georgina A.; Lambert, Sophia; Mercky, Yann; Lejeune, Mathilde; Lignot, Jehan-hervé; Sucré, Elliott. |
| Mangroves are tidal wetlands that are often under strong anthropogenic pressures, despite the numerous ecosystem services they provide. Pollution from urban runoffs is one such threats, yet some mangroves are used as a bioremediation tool for wastewater (WW) treatment. This practice can impact mangrove crabs, which are key engineer species of the ecosystem. Using an experimental area with controlled WW releases, this study aimed to determine from an ecological and ecotoxicological perspective, the effects of WW on the red mangrove crab Neosarmatium africanum. Burrow density and salinity levels (used as a proxy of WW dispersion) were recorded, and a 3-week caging experiment was performed. Hemolymph osmolality, gill Na+/K+-ATPase (NKA) activity and gill... |
| Tipo: Text |
Palavras-chave: Mangrove; Bioturbation; Wastewater; Ecophysiology; Oxidative stress; Osmoregulation. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00592/70449/68559.pdf |
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